Mysterious malware that plays a sound file and cannot be stopped or removed

I believe I have some sort of malware that automatically plays a sound file.  The sound file is either some sort of infomercial or podcast.  There is no application running and I do not no where this sound file is be played from. I disconnected my internet connection and the sound still plays so I think the file is already embedded somewhere. I have run the anti-virus and malware remover but it has not gotten rid of the problem.  I sound file begins to play out of nowhere and I cannot stop it or remove it.  Thanks!